Article
Development and validation of microarray-based assay for epidemiological study of MRSA.
Molecular and cellular probes - 1 Feb 2008
Otsuka Junnosuke, Kondoh Yasumitsu, Amemiya Tomoyuki, Kitamura Akio, Ito Teruyo, Baba Satoshi, Cui Longzhu, Hiramatsu Keiichi, Tashiro Tomoko, Tashiro Hideo
Abstract excerpt
We have developed a microarray-based assay for the genotyping of Staphylococcus aureus strains. A DNA microarray consisting of 221 genes with 390 oligonucleotide probes was designed to identify characteristic genes or gene alleles of S. aureus. The 221 genes were chosen on the basis of the following criteria: (i) genes used as control for the microarray system, (ii) virulence genes, (iii) resistance genes and...
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