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Article

Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species

1 Aug 1991

Abstract excerpt

Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization, hybridized DNAs were quantitatively detected with peroxidase-conjugated streptavidin and the...

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PMID: 1761680
DOI: 10.1128/jcm.29.8.1596-1603.1991

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