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Article

Split single-cell RT-PCR analysis of Purkinje cells.

Nature protocols - 1 Jan 2006

Esumi Shigeyuki, Kaneko Ryosuke, Kawamura Yoshimi, Yagi Takeshi

Abstract excerpt

This protocol details a method for analyzing the expression of multiple genes from a single Purkinje neuron, including the determination of whether the gene expression is monoallelic or biallelic. The protocol describes how to extract a single, living Purkinje cell for reverse transcription, divi...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 17487206
DOI: 10.1038/nprot.2006.343

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17487206-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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