Article
Whole-genome analysis of the chromosome partitioning and sporulation protein Spo0J (ParB) reveals spreading and origin-distal sites on the Bacillus subtilis chromosome.
Molecular microbiology - 1 May 2007
Breier Adam M, Grossman Alan D
Abstract excerpt
We investigated the genome-wide DNA binding of the chromosome partitioning and sporulation protein and ParB family member Spo0J in Bacillus subtilis using chromatin immunoprecipitation and DNA microarrays. We identified 10 parS loci to which Spo0J binds, two of which were unexpectedly distant (> 1 Mb) from the origin of replication. We used all 10 sites to refine the consensus sequence for parS. We found that...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Chromatin Immunoprecipitation
- Chromosomes, Bacterial
- DNA Replication
- Electrophoretic Mobility Shift Assay
- Genome, Bacterial
- Microscopy, Fluorescence
- Mutation
- Oligonucleotide Array Sequence Analysis
- Protein Binding
- Transcription, Genetic