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Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp.

Extremophiles : life under extreme conditions - 1 Jul 2007

Kageyama Yasushi, Takaki Yoshihiro, Shimamura Shigeru, Nishi Shinro, Nogi Yuichi, Uchimura Kohsuke, Kobayashi Tohru, Hitomi Jun, Ozaki Katsuya, Kawai Shuji, Ito Susumu, Horikoshi Koki

Abstract excerpt

Alkaliphilic Bacillus sp. strain KSM-K16, which produces high-alkaline M-protease, was characterized phenotypically, biochemically and genetically. This strain was identified as Bacillus clausii based on the results of taxonomic studies, including sequencing of the 16S rRNA gene and DNA-DNA hybridization. Seven rRNA operons in the genome were identified by pulsed-field gel electrophoresis. Sequencing of cloned...

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PMID: 17429572
DOI: 10.1007/s00792-007-0074-1

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