Article
Subtraction of background damage in PFGE experiments on DNA fragment-size distributions.
Radiation and environmental biophysics - 1 Jun 2007
Ponomarev Artem L, Belli Mauro, Hahnfeldt Philip J, Hlatky Lynn, Sachs Rainer K, Cucinotta Francis A
Abstract excerpt
The non-random distribution of DNA breakage in pulsed-field gel electrophoresis (PFGE) experiments poses a problem of proper subtraction of the background damage to obtain a fragment-size distribution due to radiation only. As been pointed out by various authors, a naive bin-to-bin subtraction of the background signal will not result in the right DNA mass distribution histogram, and may even result in negative...
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