An upstream region in the first intron of petunia actin-depolymerizing factor 1 affects tissue-specific expression in transgenic Arabidopsis (Arabidopsis thaliana).

Abstract excerpt

The first intron of the petunia actin-depolymerizing factor 1 (PhADF1) gene was previously shown to induce strong and constitutive expression of that gene in vegetative tissues of transgenic Arabidopsis. To examine intron-mediated enhancement of PhADF1 gene expression in detail, the effects of splicing, deletion and promoter alteration on gene expression were analyzed in this study. Deletion of the 5' upstream...

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