Article
Single nucleotide polymorphisms in rye (Secale cereale L.): discovery, frequency, and applications for genome mapping and diversity studies.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Apr 2007
Varshney R K, Beier U, Khlestkina E K, Kota R, Korzun V, Graner A, Börner A
Abstract excerpt
To elucidate the potential of single nucleotide polymorphism (SNP) markers in rye, a set of 48 barley EST (expressed sequence tag) primer pairs was employed to amplify from DNA prepared from five rye inbred lines. A total of 96 SNPs and 26 indels (insertion-deletions) were defined from the sequences of 14 of the resulting amplicons, giving an estimated frequency of 1 SNP per 58 bp and 1 indel per 214 bp in the...
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