Article
Identification of genes differentially expressed in RAW264.7 cells infected by Salmonella typhimurium using PCR method.
Journal of microbiology (Seoul, Korea) - 1 Feb 2007
Kang Kyung Ho, Song Jung A, Shin Dong-Jun, Choy Hyon E, Hong Yeongjin
Abstract excerpt
Salmonella typhimurium, causing mouse typhoid, infects hosts such as macrophage cells, and proliferates in intracellular vacuoles causing infected cells to trigger numerous genes to respond against the infection. In this study, we tried to identify such genes in RAW264.7 cells by using the PCR screening method with degenerate primers. Fourteen genes were found to be differentially expressed after a 4 h infection...
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