Article
Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio cholerae.
Journal of medical microbiology - 1 Mar 2007
Ramachandran Dhanya, Bhanumathi R, Singh Durg V
Abstract excerpt
This study describes a multiplex PCR assay for the detection of antibiotic resistance genes and the SXT element in Vibrio cholerae. Conditions were optimized to amplify fragments of sulII (encoding sulfamethoxazole resistance), dfrA1 (O1-specific trimethoprim resistance), dfr18 (O139-specific trimethoprim resistance), strB (streptomycin B resistance) and the SXT element simultaneously in one PCR. This multiplex...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
