Article
Molecular characterization of mutations affecting expression level and growth rate-dependent regulation of the Escherichia coli zwf gene.
Journal of bacteriology - 1 Jan 1992
Rowley D L, Fawcett W P, Wolf R E
Abstract excerpt
We characterized three cis dominant mutations which elevate glucose 6-phosphate dehydrogenase level. Growth rate-dependent regulation and oxidative stress control of enzyme level were altered by the mutations. DNA sequencing and transcript mapping showed that the "up" mutations created new promoters whose hyperactive expression overrides the normal regulation of the native promoter.
Topics
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Glucosephosphate Dehydrogenase
- Molecular Sequence Data
- Mutation
- Software
- Transcription, Genetic