Article
Identification and functional analysis of a delta-6 desaturase from the marine microalga Glossomastix chrysoplasta.
Marine biotechnology (New York, N.Y.) - 1 Jan 2000
Hsiao Tracy Y, Holmes Bradley, Blanch Harvey W
Abstract excerpt
A delta-6 (Delta6) desaturase gene was isolated from the marine microalga Glossomastix chrysoplasta, a stramenopile that produces large amounts of eicosapentaenoic acid (EPA). A functional analysis of this gene was performed in Saccharomyces cerevisiae. Isolation of the Delta6 fatty acid desaturase was achieved via reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed from...
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