Article
Cloning of a gene for chloroplast omega6 desaturase of a green alga, Chlamydomonas reinhardtii.
Journal of biochemistry - 1 Dec 1997
Sato N, Fujiwara S, Kawaguchi A, Tsuzuki M
Abstract excerpt
A gene for chloroplast omega6 desaturase, which catalyzes the desaturation of monoenoic to dienoic acids in chloroplasts, was isolated from Chlamydomonas reinhardtii. We first performed reverse transcriptase-polymerase chain reaction with oligonucleotide primers corresponding to regions conserved among plastid omega6 desaturases of higher plants and delta12 desaturases of cyanobacteria, using C. reinhardtii...
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