Article
PCR-RFLP of ITS rDNA for the rapid identification of Penicillium subgenus Biverticillium species.
Revista iberoamericana de micologia - 1 Sept 2006
Dupont Jöelle, Dennetière Bruno, Jacquet Claire, Dupont Marie France
Abstract excerpt
RFLP of ITS rDNA is proposed as a useful tool for molecular identification of the most common species of biverticillate penicillia. 60 isolates were analysed representing 13 species and 21 unique sequences were produced. The combination of five restriction enzymes was successful in separating 12 species. However, the variety Penicillium purpurogenum var. rubrisclerotium remained indistinguishable from Penicillium...
Topics
- DNA, Fungal
- DNA, Ribosomal
- DNA, Ribosomal Spacer
- Penicillium
- Phenotype
- Phylogeny
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Ribotyping
- Sensitivity and Specificity
- Species Specificity