Article
Isolation and characterization of hematopoietic progenitor cells in canine bone marrow.
Veterinary immunology and immunopathology - 15 Feb 2007
Wijewardana Viskam, Sugiura Kikuya, Shigeyama Naoyoshi, Moriguchi Mariko, Tsunoda Shigeru, Ikehara Susumu, Inaba Toshio
Abstract excerpt
For ultimate diagnoses of canine leukemia or malignant lymphoma, we sought to isolate hematopoietic progenitor cells (HPCs) from canine bone marrow (BM) using physiological phenotypes. Canine BM cells were separated by equilibrium discontinued density centrifugation, and HPCs, detected by in vitro colony formation, were significantly enriched in the relatively low density (LD) fraction. In flow cytometry, many...
Topics
- Animals
- Antigens, CD34
- Bone Marrow Cells
- Cell Fractionation
- Dogs
- Female
- Flow Cytometry
- Fluorescent Dyes
- Hematopoietic Stem Cells
- Histocompatibility Antigens Class II
- Multipotent Stem Cells