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Article

Detection of allele-specific transcripts by the polymerase chain reaction (AST-PCR).

Biochemical and biophysical research communications - 15 Aug 1991

Top B, van den Boorn N, van der Zee A, Havekes L M, Frants R R

Abstract excerpt

We have applied the polymerase chain reaction to detect differences in relative amount of allele-specific transcripts of the low density lipoprotein receptor gene in individuals with heterozygous familial hypercholesterolemia (FH). This method is based on detection of loss of heterozygosity of exon-specific restriction fragment length polymorphisms present in amplified mRNA fragments as compared to amplified...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 1714719
DOI: 10.1016/0006-291x(91)91038-e

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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