Article
Detection of allele-specific transcripts by the polymerase chain reaction (AST-PCR).
Biochemical and biophysical research communications - 15 Aug 1991
Top B, van den Boorn N, van der Zee A, Havekes L M, Frants R R
Abstract excerpt
We have applied the polymerase chain reaction to detect differences in relative amount of allele-specific transcripts of the low density lipoprotein receptor gene in individuals with heterozygous familial hypercholesterolemia (FH). This method is based on detection of loss of heterozygosity of ex...
Topics
- Alleles
- Base Sequence
- DNA
- Genetic Carrier Screening
- Humans
- Hyperlipoproteinemia Type II
- Leukocytes
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- RNA
- RNA, Messenger
- Receptors, LDL
- Reference Values
- Restriction Mapping
- Transcription, Genetic