Transmembrane Molecules for Phylogenetic Analyses of Pathogenic Protists: Leishmania -Specific Informative Sites in Hydrophilic Loops of Trans- Endoplasmic Reticulum N -Acetylglucosamine-1-Phosphate Transferase

Abstract excerpt

A sequence database was created for the Leishmania N-acetylglucosamine-1-phosphate transferase (nagt) gene from 193 independent isolates. PCR products of this single-copy gene were analyzed for restriction fragment length polymorphism based on seven nagt sequences initially available. We subsequently sequenced 77 samples and found 19 new variants (genotypes). Alignment of all 26 nagt sequences is gap free, except...

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