Article
[DNA amplification of Plasmodium vivax parasites from Giemsa-stained blood smears].
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 1 Aug 2006
Xiao Fang-zhen, Zhang Shan-ying, Xu Long-shan, Huang Jiang-hong, Xie Han-guo, Ou Yang-rong
Abstract excerpt
OBJECTIVE: To develop methods of extracting DNA from malaria parasites on Giemsa-stained blood smears. METHODS: Improved Na2HPO4 method and Chelex-100 ion-exchange technique were used to extract DNA from Giemsa-stained or unstained blood smears. Nested PCR was employed for amplification and ident...
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