Article
Analysis of RNA stability and (-) strand content in viral infections using biotinylated RNA probes.
Journal of virological methods - 1 Dec 1990
Rao A L, Huntley C C, Marsh L E, Hall T C
Abstract excerpt
Non-radioactive biotinylated RNA probes specific for plus (+) and minus (-) sense RNAs of brome mosaic virus (BMV) were synthesized in vitro from a plasmid bearing a 200 base pair fragment complementary to the 3' terminus of each of the three genomic RNAs of the virus. Using virion RNAs isolated from BMV infected barley plants, the sensitivity of biotinylated RNAs as hybridization probes was compared with that of...
Topics
- Biotin
- Blotting, Northern
- DNA, Viral
- Densitometry
- Mosaic Viruses
- Mutation
- Plants
- Plasmids
- Protoplasts
- RNA
- RNA Probes
- RNA, Complementary