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Enhanced ascertainment of microchimerism with real‐time quantitative polymerase chain reaction amplification of insertion‐deletion polymorphisms

27 Oct 2006

Abstract excerpt

BACKGROUND: The characterization of microchimerism (MC) by gene amplification has been limited by few allogeneic markers, ascertainment bias, and assay analytic performance. To address this, a panel of 12 MC assays based on insertion-deletion (InDel) polymorphisms had been optimized. STUDY DESIGN AND METHODS: The InDel assays were validated with comprehensive in vitro spiking studies at the stochastic limit of...

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PMID: 17076840
DOI: 10.1111/j.1537-2995.2006.00992.x

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