Article
Mutation in a "tesB-like" hydroxyacyl-coenzyme A-specific thioesterase gene causes hyperproduction of extracellular polyhydroxyalkanoates by Alcanivorax borkumensis SK2.
Journal of bacteriology - 1 Dec 2006
Sabirova Julia S, Ferrer Manuel, Lünsdorf Heinrich, Wray Victor, Kalscheuer Rainer, Steinbüchel Alexander, Timmis Kenneth N, Golyshin Peter N
Abstract excerpt
A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important polymer when grown on alkanes. The tesB-like gene encodes a distinct novel enzyme activity, which...
Topics
- Acyl Coenzyme A
- Alkanes
- Biotechnology
- Culture Media
- DNA Transposable Elements
- Escherichia coli
- Gammaproteobacteria
- Microscopy, Electron, Scanning
- Mutation
- Polyesters