Article
Molecular detection of HIV-1 subtype B, CRF01_AE, CRF33_01B, and newly emerging recombinant lineages in Malaysia.
The American journal of tropical medicine and hygiene - 1 Mar 2015
Chook Jack Bee, Ong Lai Yee, Takebe Yutaka, Chan Kok Gan, Choo Martin, Kamarulzaman Adeeba, Tee Kok Keng
Abstract excerpt
A molecular genotyping assay for human immunodeficiency virus type 1 (HIV-1) circulating in Southeast Asia is difficult to design because of the high level of genetic diversity. We developed a multiplex real-time polymerase chain reaction (PCR) assay to detect subtype B, CRF01_AE, CRF33_01B, and three newly described circulating recombinant forms, (CRFs) (CRF53_01B, CRF54_01B, and CRF58_01B). A total of 785...
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