Article
Scintillation proximity assay for DNA binding by human p53.
BioTechniques - 1 Sept 2006
Gal Susannah, Cook Jeffery R, Howells Leighton
Abstract excerpt
Many DNA binding proteins are known to regulate gene expression. When that binding is altered, a disease state can result. A common method for measuring DNA binding, namely electrophoretic mobility shift assay (EMSA) is often used but it is not amenable to rapid screening of many samples. As an alternative method, we have developed a DNA binding assay for the tumor suppressor protein p53 in a 96-well microtiter...
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