Article
Mutations within the catalytic motif of DNA adenine methyltransferase (Dam) of Aeromonas hydrophila cause the virulence of the Dam-overproducing strain to revert to that of the wild-type phenotype.
Infection and immunity - 1 Oct 2006
Erova Tatiana E, Fadl Amin A, Sha Jian, Khajanchi Bijay K, Pillai Lakshmi L, Kozlova Elena V, Chopra Ashok K
Abstract excerpt
In this study, we demonstrated that the methyltransferase activity associated with Dam was essential for attenuation of Aeromonas hydrophila virulence. We mutated aspartic acid and tyrosine residues to alanine within the conserved DPPY catalytic motif of Dam and transformed the pBAD/damD/A, pBAD/damY/A, and pBAD/damAhSSU (with the native dam gene) recombinant plasmids into the Escherichia coli GM33...
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