Article
Colony formation of clone-sorted human hematopoietic progenitors.
Blood - 15 May 1990
Ema H, Suda T, Miura Y, Nakauchi H
Abstract excerpt
To characterize human hematopoietic progenitors, we performed methylcellulose cultures of single cells isolated from a population of CD34+ cells by fluorescence-activated cell-sorting (FACS) clone-sorting system. CD34+ cells were detected in bone marrow (BM) and peripheral blood (PB) cells at incidences of 1.0% and 0.01% of total mononuclear cells, respectively. Single cell cultures revealed that approximately...
Topics
- Antigens, CD34
- Antigens, Differentiation
- Blood Cells
- Bone Marrow
- Bone Marrow Cells
- Cell Division
- Cell Separation
- Colony-Stimulating Factors
- Flow Cytometry
- Granulocyte Colony-Stimulating Factor
- Granulocyte-Macrophage Colony-Stimulating Factor
