Article
Colony formation of clone-sorted human hematopoietic progenitors.
Blood - 15 May 1990
Ema H, Suda T, Miura Y, Nakauchi H
Abstract excerpt
To characterize human hematopoietic progenitors, we performed methylcellulose cultures of single cells isolated from a population of CD34+ cells by fluorescence-activated cell-sorting (FACS) clone-sorting system. CD34+ cells were detected in bone marrow (BM) and peripheral blood (PB) cells at incidences of 1.0% and 0.01% of total mononuclear cells, respectively. Single cell cultures revealed that approximately...
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