The use of anti-CD3 and anti-CD28 monoclonal antibodies to clone and expand human antigen-specific T cells.

doi:10.1016/0022-1759(90)90210-m

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Article

The use of anti-CD3 and anti-CD28 monoclonal antibodies to clone and expand human antigen-specific T cells.

Journal of immunological methods - 17 Apr 1990

Riddell S R, Greenberg P D

Abstract excerpt

Antigen-specific T cell clones are useful reagents for studies of the fine specificity of antigen recognition and of potential therapeutic use in adoptive immunotherapy for human viral and malignant diseases. Culture methods which require antigen and APC for stimulation can be problematic for the generation and long-term growth of human virus and tumor-specific T cells. We have developed an alternative culture...

Topics

Antibodies, Monoclonal
Antigens, Differentiation, T-Lymphocyte
Antigens, Surface
CD28 Antigens
CD3 Complex
Cell Division
Cell Line, Transformed
Cells, Cultured
Clone Cells
Cytomegalovirus
Epitopes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Immunologic Techniques

Lymphocyte Activation

Lymphocyte Culture Test, Mixed

Phenotype

Receptors, Antigen, T-Cell

T-Lymphocytes

Source

Open in PubMed

Identifiers

PMID: 1691237
DOI: 10.1016/0022-1759(90)90210-m

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1691237-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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