Article
Mutation breeding of chitosanase-producing strain Bacillus sp. S65 by low-energy ion implantation.
Journal of industrial microbiology & biotechnology - 1 Dec 2006
Su Caixin, Zhou Wei, Fan Yonghong, Wang Li, Zhao Shiguang, Yu Zengliang
Abstract excerpt
In order to obtain an industrial strain with higher chitosanase yield, the wild strain Bacillus sp. S65 cells were mutated by a novel mutagen, nitrogen ion beam, with energy of 15 keV and dose ranging from 2.6 x 10(14 )to 5.2 x 10(15) ions/cm(2). One mutant, s65F5 with high yield of chitosanase was isolated. Results showed that the production of chitosanase of s65F5 was dramatically increased from 4.1 U/ml in s65...
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