Article
Comparison of 12 reference genes for normalization of gene expression levels in Epstein-Barr virus-transformed lymphoblastoid cell lines and fibroblasts.
Molecular diagnosis & therapy - 1 Jan 2006
de Brouwer Arjan P M, van Bokhoven Hans, Kremer Hannie
Abstract excerpt
BACKGROUND: Epstein-Barr virus-transformed lymphoblastoid cell lines (EBV-LCLs) and skin fibroblasts are often used to establish or confirm the effect of mutations on gene expression levels. Relative quantification of gene expression is usually achieved by comparison of the expression level of a gene of interest with that of a suitable reference gene. Hence, the choice of reference gene is critical in all...
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