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Detection of fish nocardiosis by loop-mediated isothermal amplification

21 Apr 2006

Abstract excerpt

AIMS: Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for detecting Nocardia seriolae which is a causative agent of fish nocardiosis, was designed. METHODS AND RESULTS: A set of four primers, two inner and two outer, were designed based on the sequence of the 16S-23S...

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PMID: 16696687
DOI: 10.1111/j.1365-2672.2006.02872.x

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