Article
Genotypic identification of presumptive Streptococcus pneumoniae by PCR using four genes highly specific for S. pneumoniae.
Journal of medical microbiology - 1 Jun 2006
Suzuki Nao, Yuyama Mayumi, Maeda Sinsaku, Ogawa Haruhiko, Mashiko Kazuyuki, Kiyoura Yusuke
Abstract excerpt
It was previously reported that two oligonucleotide primer sets (spn9802 and spn9828) for discriminating Streptococcus pneumoniae from pneumococcus-like oral streptococcal isolates using PCR had been developed. In this study, PCR amplification of the lytA, ply, spn9802 and spn9828 genes was used to identify presumptive S. pneumoniae. Two genetic groups were identified by analysing sputum samples from 28 patients...
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