Article
Phage abortive infection mechanism from Lactococcus lactis subsp. lactis, expression of which is mediated by an Iso-ISS1 element.
Applied and environmental microbiology - 1 Dec 1991
Cluzel P J, Chopin A, Ehrlich S D, Chopin M C
Abstract excerpt
A 5-kb DNA fragment conferring a phage abortive infection phenotype (Abi+) has been cloned from Lactococcus lactis subsp. lactis IL416. The Abi+ determinant was subcloned on a 2-kb fragment which carried an Iso-ISS1 element and an open reading frame of 753 bp designated ORFX. Deletion within ORFX entailed the loss of the Abi+ phenotype, establishing that ORFX is the structural abi-416 gene. The expression of...
Topics
- Amino Acid Sequence
- Bacteriophages
- Base Sequence
- Cloning, Molecular
- DNA Transposable Elements
- DNA, Bacterial
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Kinetics
- Lactococcus lactis