In vitro transcription amplification and labeling methods contribute to the variability of gene expression profiling with DNA microarrays.

Abstract excerpt

The effect of different amplification and labeling methods on DNA microarray expression results has not been previously delineated. To analyze the variation associated with widely accepted T7-based RNA amplificationand labeling methods, aliquots of the Stratagene Human Universal Reference RNA were labeled using three eukaryotic target preparation methods followed by uniform replicate array hybridization...

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