Article
dye (arc) Mutants: insights into an unexplained phenotype and its suppression by the synthesis of poly (3-hydroxybutyrate) in Escherichia coli recombinants.
FEMS microbiology letters - 1 May 2006
Ruiz Jimena A, Fernández Rubén O, Nikel Pablo I, Méndez Beatriz S, Pettinari M Julia
Abstract excerpt
arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2...
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