Article
[Variability analysis of S2 gene of SARS-CoV].
Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 1 Apr 2006
Zhou Hao, Long Bei-guo, Zhang Wen-bing, Jiang Li-fang, Chen Li-dan, Gong Shu-ji, Zhao Wei
Abstract excerpt
OBJECTIVE: To determine the sequence of S2 gene of SARS-associated coronavirus (SARS-CoV) GD322 and analyze the phyletic evolution of S2 gene. METHOD: S2 gene fragment was amplified from SARS-CoV GD322 genome with RT-PCR and ligated to pGEM-T vector for sequence analysis after transformation of the plasmid into E. coli DH5a. The variability of S2 genes and S2 proteins from 12 strains isolated in the early,...
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