Article
Definition of the bacterial N-glycosylation site consensus sequence.
The EMBO journal - 3 May 2006
Kowarik Michael, Young N Martin, Numao Shin, Schulz Benjamin L, Hug Isabelle, Callewaert Nico, Mills Dominic C, Watson David C, Hernandez Marcela, Kelly John F, Wacker Michael, Aebi Markus
Abstract excerpt
The Campylobacter jejuni pgl locus encodes an N-linked protein glycosylation machinery that can be functionally transferred into Escherichia coli. In this system, we analyzed the elements in the C. jejuni N-glycoprotein AcrA required for accepting an N-glycan. We found that the eukaryotic primary consensus sequence for N-glycosylation is N terminally extended to D/E-Y-N-X-S/T (Y, X not equalP) for recognition by...
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