Article
Recovery of a recombinant salmonid alphavirus fully attenuated and protective for rainbow trout.
Journal of virology - 1 Apr 2006
Moriette Coralie, Leberre Monique, Lamoureux Annie, Lai Thanh-Lan, Brémont Michel
Abstract excerpt
Sleeping disease virus (SDV) is a member of the new Salmonid alphavirus genus within the Togaviridae family. The single-stranded RNA genome of SDV is 11,894 nucleotides long, excluding the 3' poly(A) tail. A full-length cDNA has been generated; the cDNA was fused to a hammerhead ribozyme sequence at the 5' end and inserted into a transcription plasmid (pcDNA3) backbone, yielding pSDV. By transfection of pSDV into...
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