Article
A cDNA encoding rabbit muscle protein phosphatase 1 alpha complements the Aspergillus cell cycle mutation, bimG11.
The Journal of biological chemistry - 5 Oct 1991
Doonan J H, MacKintosh C, Osmani S, Cohen P, Bai G, Lee E Y, Morris N R
Abstract excerpt
The bimG11 allele causes a conditional growth defect in the fungus Aspergillus nidulans preventing both progression through mitosis and normal polar growth. Previously, we have shown that the bimG11 mutation increases the phosphorylation of nuclear proteins and that the gene encodes a protein similar to mammalian type 1 protein phosphatase. Assay of protein phosphatase activity in protein extracts of Aspergillus...
Topics
- Alleles
- Animals
- Aspergillus
- Blotting, Southern
- Cell Cycle
- Cloning, Molecular
- Ethers, Cyclic
- Genetic Complementation Test
- Kinetics
- Muscles
- Mutation