Article
Improvement of Potato virus Y (PVY) detection and quantitation using PVY(N)- and PVY(O)-specific real-time RT-PCR assays.
Journal of virological methods - 1 Jun 2006
Balme-Sinibaldi Valérie, Tribodet Michel, Croizat Flora, Lefeuvre Pierre, Kerlan Camille, Jacquot Emmanuel
Abstract excerpt
A Potato virus Y (PVY) single nucleotide polymorphism (A/G(2213)), recently identified as a molecular determinant of the tobacco leaf necrosis symptom induced by PVY(N) isolates, has been used as a target to develop two PVY group-specific (PVY(N) and PVY(O)) fluorescent (TaqMan-based) real-time RT-PCR assays. These procedures allow detection, characterisation, and quantitation of a wide range of PVY isolates in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
