Article
Variability of Potato virus Y in Tomato Crops in Poland and Development of a Reverse-Transcription Loop-Mediated Isothermal Amplification Method for Virus Detection.
Phytopathology - 1 Sept 2015
Hasiów-Jaroszewska Beata, Stachecka Joanna, Minicka Julia, Sowiński Mateusz, Borodynko Natasza
Abstract excerpt
A collection of 147 Potato virus Y (PVY) isolates from tomato, originating from several commercial fields and greenhouses in different regions of Poland, was tested for the presence of PVY by reverse-transcription polymerase chain reaction. However, in some cases, the results obtained were ambiguous. Therefore, a sensitive reverse-transcription loop-mediated isothermal amplification method was developed for rapid...
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