Article
Acid phosphatase-1(1), a tightly linked molecular marker for root-knot nematode resistance in tomato: from protein to gene, using PCR and degenerate primers containing deoxyinosine.
Plant molecular biology - 1 Apr 1991
Aarts J M, Hontelez J G, Fischer P, Verkerk R, van Kammen A, Zabel P
Abstract excerpt
With a view to cloning the root-knot nematode resistance gene Mi in tomato by chromosome walking, we have developed a molecular probe for the tightly linked acid phosphatase-1 (Aps-1) locus. The acid phosphatase-1 allozyme (APS-1(1], encoded by the Aps-1(1) allele originating from Lycopersicon peruvianum, was purified to apparent homogeneity from tomato roots and suspension cells. Microsequencing of CNBr and...
Topics
- Acid Phosphatase
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Chromatography
- Chromatography, Ion Exchange
- Durapatite
- Electrophoresis, Polyacrylamide Gel