Article
Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells.
Nucleic acids research - 25 Jul 1991
Chapman B S, Thayer R M, Vincent K A, Haigwood N L
Abstract excerpt
A 2.4 kb fragment of hCMV (Towne strain), containing the 5' end of the major immediate-early gene, has been cloned, sequenced, and used to construct a series of mammalian cell expression plasmids. The effects of regulatory regions present on this fragment were assessed using human glycoproteins a...
Topics
- Animals
- Antigens, Viral
- Base Sequence
- Cell Line, Transformed
- Cloning, Molecular
- Cytomegalovirus
- DNA, Viral
- Enhancer Elements, Genetic
- Gene Expression Regulation, Viral
- Genes, Viral
- Glycoproteins
- Humans
- Immediate-Early Proteins
- Introns
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic