Article
Effect of intron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells.
Nucleic acids research - 25 Jul 1991
Chapman B S, Thayer R M, Vincent K A, Haigwood N L
Abstract excerpt
A 2.4 kb fragment of hCMV (Towne strain), containing the 5' end of the major immediate-early gene, has been cloned, sequenced, and used to construct a series of mammalian cell expression plasmids. The effects of regulatory regions present on this fragment were assessed using human glycoproteins as reporter molecules. We compared secreted levels of Factor VIII, t-PA, and HIV-1 envelope glycoproteins in cells...
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