Article
Human lung project: evaluating variance of gene expression in the human lung.
American journal of respiratory cell and molecular biology - 1 Jul 2006
Gruber Michael P, Coldren Christopher D, Woolum Malcolm D, Cosgrove Gregory P, Zeng Chan, Barón Anna E, Moore Mark D, Cool Carlyne D, Worthen G Scott, Brown Kevin K, Geraci Mark W
Abstract excerpt
Nondiseased tissue is an important reference for microarray studies of pulmonary disease. We obtained 23 single lungs from multiorgan donors at time of procurement. Donors varied in age, sex, smoking history, and ethnicity. Lungs were dissected into upper and lower lobe peripheral sections for RNA extraction. Microarray analysis was performed using Affymetrix Hu-133 Plus 2.0 arrays. We observed that the relative...
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