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Production of a transgenic piglet by a sperm injection technique in which no chemical or physical treatments were used for oocytes or sperm

17 Feb 2006

Abstract excerpt

As a method of producing transgenic animals, spermatozoa have been used to fertilize mammalian oocytes through natural copulation, artificial insemination (AI), and in vitro fertilization (IVF). Our objective was to produce live piglets expressing the enhanced green fluorescent protein (eGFP) by the modified ICSI procedure based on Yong et al. (2003) (Hum. Reprod. 18:2390) where this procedure resulted in an...

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PMID: 16489622
DOI: 10.1002/mrd.20477

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