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Article

Computational Methods for Analysis of Foci: Validation for Radiation-Induced γ-H2AX Foci in Human Cells

1 Jan 2006

Abstract excerpt

Observation and counting of gamma-H2AX foci in untreated cells as well as in cells exposed to cytotoxic agents is a widely used method for documenting the presence of double-strand breaks (DSBs) in the DNA and for analysis of their repair. Similar methods are employed to analyze formation of foci by a variety of proteins implicated in the cellular responses to DNA damage. Despite the wide application of the...

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PMID: 16392969
DOI: 10.1667/rr3486.1

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