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Article

Rapid Ped-2E9 cell-based cytotoxicity analysis and genotyping of Bacillus species.

Journal of clinical microbiology - 1 Dec 2005

Gray Kristen M, Banada Padmapriya P, O'Neal Erin, Bhunia Arun K

Abstract excerpt

Bacillus species causing food-borne disease produce multiple toxins eliciting gastroenteritis. Toxin assays with mammalian cell cultures are reliable but may take 24 to 72 h to complete and also lack sensitivity. Here, a sensitive and rapid assay was developed using a murine hybridoma Ped-2E9 cell model. Bacillus culture supernatants containing toxins were added to a Ped-2E9 cell line and analyzed for...

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Identifiers

PMID: 16333068
DOI: 10.1128/JCM.43.12.5865-5872.2005
PMCID: PMC1317164

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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