Antibiotic Susceptibility Testing of <i>Mycoplasma genitalium</i> by TaqMan 5′ Nuclease Real-Time PCR

Abstract excerpt

Mycoplasma genitalium is an important pathogen in male nongonococcal urethritis (NGU). Isolation of M. genitalium from clinical specimens by axenic culture is very difficult and time-consuming, and very few strains are available for antibiotic susceptibility testing. Primary isolation of M. genitalium by coculture with Vero cells improves the isolation rate significantly. However, some strains cannot be adapted...

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