Use of TaqMan 5′ Nuclease Real-Time PCR for Quantitative Detection of <i>Mycoplasma genitalium</i> DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic

Abstract excerpt

Mycoplasma genitalium is a cause of nongonococcal urethritis, particularly in patients not infected with Chlamydia trachomatis. A quantitative 5' nuclease assay (TaqMan PCR) was developed and validated. The assay detected a fragment of the MgPa adhesin gene by use of a TaqMan MGB (minor groove binder) probe and included an internal processing control to detect PCR inhibition. Urethral swab specimens and...

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