Excretion of ammonium by a nifL mutant of Azotobacter vinelandii fixing nitrogen.

Abstract excerpt

A mutation in the gene upstream of nifA in Azotobacter vinelandii was introduced into the chromosome to replace the corresponding wild-type region. The resulting mutant, MV376, produced nitrogenase constitutively in the presence of 15 mM ammonium. When introduced into a nifH-lacZ fusion strain, the mutation permitted beta-galactosidase production in the presence of ammonium. The gene upstream of nifA is therefore...

Topics

• Ammonia
• Azotobacter vinelandii
• Genes, Bacterial
• Klebsiella pneumoniae
• Molecular Sequence Data
• Mutation
• Nitrogen Fixation