Article
Analysis of the genetic diversity of Legionella by sequencing the 23S-5S ribosomal intergenic spacer region: from phylogeny to direct identification of isolates at the species level from clinical specimens.
Microbes and infection - 1 Jan 2006
Grattard Florence, Ginevra Christophe, Riffard Serge, Ros Alain, Jarraud Sophie, Etienne Jerome, Pozzetto Bruno
Abstract excerpt
This study focuses on the interest of the hypervariable 23S-5S ribosomal intergenic spacer region (ISR) of the genus Legionella to analyze the phylogenic diversity of Legionella at the species and subspecies levels and to identify isolates directly from clinical specimens. The method, using a real-time PCR assay with a single primer pair followed by sequencing, was able to identify correctly 49 reference strains...
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