Article
Unexpected elevated production of Aquifex aeolicus cytochrome c555 in Escherichia coli cells lacking disulfide oxidoreductases.
Bioscience, biotechnology, and biochemistry - 1 Jul 2005
Kojima Nobusuke, Yamanaka Masaru, Ichiki Shin-ichi, Sambongi Yoshihiro
Abstract excerpt
Mutant strains of Escherichia coli lacking DsbA, DsbB, or DsbD (proteins required for disulfide bond formation in the periplasm) did not produce mitochondrial or chloroplast cytochromes c, as previously observed for bacterial ones. Unexpectedly, however, cytochrome c(555) (AA c(555)) from a hyperthermophile, Aquifex aeolicus, was produced in the E. coli periplasm without Dsb proteins, three times more than with...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
